Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

KH Tan; CA Seers; SG Dashper; HL Mitchell; JS Pyke; V Meuric; N Slakeski; SM Cleal; JL Chambers; MJ McConville; EC Reynolds

Journal article

Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

KH Tan, CA Seers, SG Dashper, HL Mitchell, JS Pyke, V Meuric, N Slakeski, SM Cleal, JL Chambers, MJ McConville, EC Reynolds

Plos Pathogens | Published : 2014

DOI: 10.1371/journal.ppat.1003955

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Abstract

Porphyromonas gingivalis and Treponema denticola are strongly associated with chronic periodontitis. These bacteria have been co-localized in subgingival plaque and demonstrated to exhibit symbiosis in growth in vitro and synergistic virulence upon co-infection in animal models of disease. Here we show that during continuous co-culture a P. gingivalis:T. denticola cell ratio of 6:1 was maintained with a respective increase of 54% and 30% in cell numbers when compared with mono-culture. Co-culture caused significant changes in global gene expression in both species with altered expression of 184 T. denticola and 134 P. gingivalis genes. P. gingivalis genes encoding a predicted thiamine biosyn..

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University of Melbourne Researchers

Christine Seers Author

Stuart Dashper Author

Nada Slakeski Author

Malcolm McConville Author

Related Projects (1)

Bacterial interactions related to disease

In this study we will determine how three pathogenic species of bacteria interact. Together these species are associated with periodontitis ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by the Australian National Health and Medical Research Council Project Grant Number 1008061. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.